RNA-Seq from any organism using probe-free rRNA depletion

Tuesday, 27th October, 2020, 02:00 pm - 03:00 pm EDT

RNA-Seq is a powerful tool that allows researchers to effectively digitize the transcriptome of a biological sample. However, ribosomal RNA (rRNA) transcripts typically comprise between 80% and 90% of all cellular RNAs and must be eliminated from RNA-Seq libraries for researchers to efficiently analyze the remaining 10% of biologically relevant sequences.
Oligo(T)-mediated mRNA-enrichment and probe-based ribosomal depletion are the two of the most common methods used to eliminate rRNA reads from RNA-Seq libraries. Both of the methods introduce off-target bias into RNA-Seq data. Zymo Research has developed a novel probe-free rRNA depletion method which is effectively free of bias and best preserves the original transcriptome profile of a sample. We find that the probe-free method is universally applicable to any species, even across samples representing organisms from different biological kingdoms and phyla.